Click-It: Pre-targeting imaging

06.02.19

Within this animation the concept of bioorthogonal imaging involving a clearing agent is visualized. First, a TCO tagged antibody is administered and allowed for sufficient time to accumulate in target tissue. Excess antibody still in circulation is inactivated and excreted by a polymeric clearing agent. A ¹⁸F labeled tetrazine is then given, and ligates to the pre-accumulated antibody within a short time span by the tetrazine-TCO bioorthogonal ligation. Non-bound ¹⁸F-tetrazine imaging agent is rapidly excreted via the kidneys, allowing for PET imaging with high contrast.